Comparison of surface iodination methods by electron microscopic autoradiography applied in vitro to different life-stages of Dipetalonema vitae (Filarioidea)
نویسندگان
چکیده
منابع مشابه
Dipetalonema viteae (Filarioidea): development of the infective larvae in vitro.
The development of Dipetalonema viteae third stage larvae was attempted in vitro. A monophasic culture system consisting of BHK-21 medium supplemented with 10% tryptose-phosphate broth and 15% fresh jird serum allowed the growth of previously in vivo triggered larvae, but not of those isolated from ticks. The larvae could complete their third moult and grew on a fourth stage larvae up to 5 mm. ...
متن کاملElectron Microscopic Autoradiography
An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal spreading mechanism. Maize root tips grown in the presence of H(3)-thymidine were examined electro...
متن کاملElectron Microscopic Autoradiography
Iodide is concentrated from the blood by the thyroid gland; after oxidation it is incorporated in tyrosyl residues of the thyroglobulin molecule. Thus the halogen is stored as a component of thyroglobulin in the colloid of the thyroid follicle. Secretion of thyroid hormone is considered to require proteolysis of the thyroglobulin and release of iodinated thyronines from the base of the acinar c...
متن کاملQuantitative Electron Microscopic Autoradiography
Quantitative electron microscopic autoradiography was used for comparing the binding of labeled somatostatin-14 (S-14) and somatostatin-28 (S-28) to islet cells. Monolayer cultures of rat islet cells were incubated with ['251-Tyr"jS-14 (S-14) or [125I-Leu8, D-Trp22, TyreJS-28 (S-281) in the presence or absence of excess unlabeled peptides. Autoradiographic grains (ARG) associated with individua...
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ژورنال
عنوان ژورنال: Parasitology
سال: 1982
ISSN: 0031-1820,1469-8161
DOI: 10.1017/s003118200005633x